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KMID : 1161420110140030240
Journal of Medicinal Food
2011 Volume.14 No. 3 p.240 ~ p.246
Gallic Acid Inhibits Cell Viability and Induces Apoptosis in Human Monocytic Cell Line U937
Kim Nam-Seok

Jeong Seung-Il
Hwang Byung-Soon
Lee Young-Eun
Kang Shin-Ho
Lee Hyung-Chul
Oh Chan-Ho
Abstract
In the present study, we investigated the effects of gallic acid (GA) (3,4,5-trihydroxybenzoic acid), a polyhydroxyphenolic compound, isolated from Rhus chinensis, on the human monocytic lymphoma cell line U937. In vitro experiments showed that treating U937 cells with various amounts of GA inhibited cell viability and induced apoptosis in a dose-dependent manner. In order to understand the mechanism by which GA induces apoptosis, we examined the gene expression of p53, nuclear factor ¥êB (NF-¥êB), and inhibitor of NF-¥êB (I-¥êB) after treating the cells with GA and found that expression levels of the genes for p53 and NF-¥êB increased and that for I-¥êB decreased. The results obtained from western blotting with U937 cells showed up-regulation of NF-¥êB protein and down-regulation of proliferating cell nuclear antigen and I-¥êB protein. These results demonstrate that GA efficiently induces apoptosis in U937 cells and that GA is a potential chemotherapeutic agent against lymphoma.
KEYWORD
apoptosis, cell viability, gallic acid, lymphoma
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